Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses

Edward Wright; David T.S. Hayman; Aisling Vaughan; Nigel J. Temperton; James L.N. Wood; Andrew A. Cunningham; Richard Suu-Ire; Robin A. Weiss; Anthony R. Fooks

doi:10.1016/j.virol.2010.09.014

Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses

Edward Wright, David T.S. Hayman, Aisling Vaughan, Nigel J. Temperton, James L.N. Wood, Andrew A. Cunningham, Richard Suu-Ire, Robin A. Weiss, Anthony R. Fooks

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fruit bat, Eidolon helvum. Highly correlated titres were observed between single and multiplex PNAs using ptLBV and ptMOKV (r=0.97, p< 0.0001), validating its use for bat serosurveillance. Of the bat serum samples screened 56% neutralised ptLBV, 27% ptMOKV and 1% ptWCBV. Mean VNAb titres were 1:266, 1:35 and 1:7 against ptLBV, ptMOKV and ptWCBV respectively. The high seroprevalence estimates suggest that the infection rate of LBV in E. helvum remains high enough to persist in this species. This supports the hypothesis that LBV is endemic in Ghanaian E. helvum and we speculate that LBV may have co-evolved with African megachiroptera.

Original language	English
Pages (from-to)	183-189
Number of pages	7
Journal	Virology
Volume	408
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2010.09.014
Publication status	Published - 20 Dec 2010
Externally published	Yes

Keywords

Africa
Antibody
Bat
Ghana
Lagos bat virus
Lyssavirus
Neutralisation
Pseudotype
Rabies virus
Serology

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10.1016/j.virol.2010.09.014

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title = "Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses",

abstract = "It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fruit bat, Eidolon helvum. Highly correlated titres were observed between single and multiplex PNAs using ptLBV and ptMOKV (r=0.97, p< 0.0001), validating its use for bat serosurveillance. Of the bat serum samples screened 56% neutralised ptLBV, 27% ptMOKV and 1% ptWCBV. Mean VNAb titres were 1:266, 1:35 and 1:7 against ptLBV, ptMOKV and ptWCBV respectively. The high seroprevalence estimates suggest that the infection rate of LBV in E. helvum remains high enough to persist in this species. This supports the hypothesis that LBV is endemic in Ghanaian E. helvum and we speculate that LBV may have co-evolved with African megachiroptera.",

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author = "Edward Wright and Hayman, {David T.S.} and Aisling Vaughan and Temperton, {Nigel J.} and Wood, {James L.N.} and Cunningham, {Andrew A.} and Richard Suu-Ire and Weiss, {Robin A.} and Fooks, {Anthony R.}",

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AU - Wright, Edward

AU - Hayman, David T.S.

AU - Vaughan, Aisling

AU - Temperton, Nigel J.

AU - Wood, James L.N.

AU - Cunningham, Andrew A.

AU - Suu-Ire, Richard

AU - Weiss, Robin A.

AU - Fooks, Anthony R.

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AB - It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fruit bat, Eidolon helvum. Highly correlated titres were observed between single and multiplex PNAs using ptLBV and ptMOKV (r=0.97, p< 0.0001), validating its use for bat serosurveillance. Of the bat serum samples screened 56% neutralised ptLBV, 27% ptMOKV and 1% ptWCBV. Mean VNAb titres were 1:266, 1:35 and 1:7 against ptLBV, ptMOKV and ptWCBV respectively. The high seroprevalence estimates suggest that the infection rate of LBV in E. helvum remains high enough to persist in this species. This supports the hypothesis that LBV is endemic in Ghanaian E. helvum and we speculate that LBV may have co-evolved with African megachiroptera.

KW - Africa

KW - Antibody

KW - Bat

KW - Ghana

KW - Lagos bat virus

KW - Lyssavirus

KW - Neutralisation

KW - Pseudotype

KW - Rabies virus

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ER -

Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses

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Keywords

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