The basal proton conductance of mitochondria depends on adenine nucleotide translocase content

Martin D. Brand, Julian L. Pakay, Augustine Ocloo, Jason Kokoszka, Douglas C. Wallace, Paul S. Brookes, Emma J. Cornwall

Research output: Contribution to journalArticlepeer-review

313 Citations (Scopus)

Abstract

The basal proton conductance of mitochondria causes mild uncoupling and may be an important contributor to metabolic rate. The molecular nature of the proton-conductance pathway is unknown. We show that the proton conductance of muscle mitochondria from mice in which isoform 1 of the adenine nucleotide translocase has been ablated is half that of wild-type controls. Overexpression of the adenine nucleotide translocase encoded by the stress-sensitive B gene in Drosophila mitochondria increases proton conductance, and underexpression decreases it, even when the carrier is fully inhibited using carboxyatractylate. We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions.

Original languageEnglish
Pages (from-to)353-362
Number of pages10
JournalBiochemical Journal
Volume392
Issue number2
DOIs
Publication statusPublished - 1 Dec 2005
Externally publishedYes

Keywords

  • Adenine nucleotide translocase (ANT) knock-out mouse
  • Carboxyatractylate
  • Drosophila
  • Proton leak

Fingerprint

Dive into the research topics of 'The basal proton conductance of mitochondria depends on adenine nucleotide translocase content'. Together they form a unique fingerprint.

Cite this