T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix, GSK) vaccine strain

Asha A. Philip; Chantal A. Agbemabiese; Guanghui Yi; John T. Patton

doi:10.1128/MRA.00603-23

T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix, GSK) vaccine strain

Asha A. Philip
, Chantal A. Agbemabiese
, Guanghui Yi
, John T. Patton

Indiana University Bloomington
University of Ghana

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.

Original language	English
Journal	Genome Announcements
Volume	12
Issue number	11
DOIs	https://doi.org/10.1128/MRA.00603-23
Publication status	Published - 2023
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

RIX4414 virus
recombinant virus
reverse genetic analysis
rotavirus
vaccines

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10.1128/MRA.00603-23

Cite this

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title = "T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix, GSK) vaccine strain",

abstract = "The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.",

keywords = "RIX4414 virus, recombinant virus, reverse genetic analysis, rotavirus, vaccines",

author = "Philip, \{Asha A.\} and Agbemabiese, \{Chantal A.\} and Guanghui Yi and Patton, \{John T.\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2023 Philip et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.",

year = "2023",

doi = "10.1128/MRA.00603-23",

language = "English",

volume = "12",

journal = "Genome Announcements",

issn = "2169-8287",

number = "11",

}

TY - JOUR

T1 - T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix, GSK) vaccine strain

AU - Philip, Asha A.

AU - Agbemabiese, Chantal A.

AU - Yi, Guanghui

AU - Patton, John T.

PY - 2023

Y1 - 2023

N2 - The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.

AB - The live oral rotavirus RV1 (Rotarix) vaccine is formulated from the human G1P[8] RIX4414 virus. Based on RIX4414 sequences, T7 expression plasmids were constructed that supported recovery of recombinant RIX4414-like viruses by reverse genetics. These plasmids will advance the study of the RV1 vaccine, possibly allowing improvements to its efficacy.

KW - RIX4414 virus

KW - recombinant virus

KW - reverse genetic analysis

KW - rotavirus

KW - vaccines

UR - https://www.scopus.com/pages/publications/85177240671

U2 - 10.1128/MRA.00603-23

DO - 10.1128/MRA.00603-23

M3 - Article

AN - SCOPUS:85177240671

SN - 2169-8287

VL - 12

JO - Genome Announcements

JF - Genome Announcements

IS - 11

ER -

T7 expression plasmids for producing a recombinant human G1P[8] rotavirus comprising RIX4414 sequences of the RV1 (Rotarix, GSK) vaccine strain

Abstract

UN SDGs

Keywords

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