TY - JOUR
T1 - Systemic suppression of interferon-γ responses in Buruli ulcer patients resolves after surgical excision of the lesions caused by the extracellular pathogen Mycobacterium ulcerans
AU - Yeboah-Manu, Dorothy
AU - Peduzzi, Elisabetta
AU - Mensah-Quainoo, Ernestina
AU - Asante-Poku, Adwoa
AU - Ofori-Adjei, David
AU - Pluschke, Gerd
AU - Daubenberger, Claudia A.
PY - 2006/6
Y1 - 2006/6
N2 - Buruli ulcer (BU), caused by Mycobacterium ulcerans, is the third most common mycobacterial infection in immunocompetent humans besides tuberculosis and leprosy. We have compared by ex vivo enzyme-linked immunospot analysis interferon-γ (IFN-γ) responses in peripheral blood mononuclear cells (PBMC) from BU patients, household contacts, and individuals living in an adjacent M. ulcerans nonendemic region. PBMC were stimulated with purified protein derivative (PPD) and nonmycobacterial antigens such as reconstituted influenza virus particles and isopentenyl-pyrophosphate. With all three antigens, the number of IFN-γ spot-forming units was reduced significantly in BU patients compared with the controls from a nonendemic area. This demonstrates for the first time that M. ulcerans infection-associated systemic reduction in IFN-γ responses is not confined to stimulation with live or dead mycobacteria and their products but extends to other antigens. Interleukin (IL)-12 secretion by PPD-stimulated PBMC was not reduced in BU patients, indicating that reduction in IFN-γ responses was not caused by diminished IL-12 production. Several months after surgical excision of BU lesions, IFN-γ responses of BU patients against all antigens used for stimulation recovered significantly, indicating that the measured systemic immunosuppression was not the consequence of a genetic defect in T cell function predisposing for BU but is rather related to the presence of M. ulcerans bacteria.
AB - Buruli ulcer (BU), caused by Mycobacterium ulcerans, is the third most common mycobacterial infection in immunocompetent humans besides tuberculosis and leprosy. We have compared by ex vivo enzyme-linked immunospot analysis interferon-γ (IFN-γ) responses in peripheral blood mononuclear cells (PBMC) from BU patients, household contacts, and individuals living in an adjacent M. ulcerans nonendemic region. PBMC were stimulated with purified protein derivative (PPD) and nonmycobacterial antigens such as reconstituted influenza virus particles and isopentenyl-pyrophosphate. With all three antigens, the number of IFN-γ spot-forming units was reduced significantly in BU patients compared with the controls from a nonendemic area. This demonstrates for the first time that M. ulcerans infection-associated systemic reduction in IFN-γ responses is not confined to stimulation with live or dead mycobacteria and their products but extends to other antigens. Interleukin (IL)-12 secretion by PPD-stimulated PBMC was not reduced in BU patients, indicating that reduction in IFN-γ responses was not caused by diminished IL-12 production. Several months after surgical excision of BU lesions, IFN-γ responses of BU patients against all antigens used for stimulation recovered significantly, indicating that the measured systemic immunosuppression was not the consequence of a genetic defect in T cell function predisposing for BU but is rather related to the presence of M. ulcerans bacteria.
KW - Ex vivo ELISpot analysis
KW - Immunosuppression
KW - Interleukin-12
UR - http://www.scopus.com/inward/record.url?scp=33746879601&partnerID=8YFLogxK
U2 - 10.1189/jlb.1005581
DO - 10.1189/jlb.1005581
M3 - Article
C2 - 16531561
AN - SCOPUS:33746879601
SN - 0741-5400
VL - 79
SP - 1150
EP - 1156
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 6
ER -