TY - JOUR
T1 - Study on the functional properties of potential indigenous probiotics isolated from human samples in West Africa
AU - Fredua-Agyeman, Mansa
AU - Ofosu-Boateng, Malvin
AU - Offei, Adelaide Ohenasi
AU - Mehta, Mausam
AU - Gaisford, Simon
AU - Limayem, Alya
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/11
Y1 - 2020/11
N2 - A study was performed to isolate and evaluate potential indigenous probiotic strains in Ghana. A total of 99 strains were isolated from human breast milk (n = 29) and fecal samples (n = 70), which were identified and characterized using methods ranging from plating growth tests and presumptive analysis at species level using MALDI-TOF MS, prior to validation through 16S rRNA gene sequence analysis. Gastrointestinal tolerance and ability to form biofilms in vitro were determined. Results indicated that out of 99 isolates, 25 were Gram-positive, catalase-negative rods. More than one-third was identified as Lactobacillus fermentum. Others were identified to be Lactobacillus (plantarum, rhamnosus, salivarius, reuteri), Enterococcus faecium, Weissella spp. and Pediococcus spp. Among the 25 isolates, 9 had activity against both Gram-negative and Gram-positive pathogens including reference or local clinical isolates of Pseudomonas aeruginosa, Salmonella Typhi, Staphylococcus aureus and Escherichia coli. Almost all isolates were effective against P. aeruginosa, E. coli and S. Typhi. Thirteen isolates did not show activity towards S. aureus. The isolates were more effective against local pathogens tested than nonindigenous pathogens. The data showed survival of all studied isolate at pH 2, 3 and 6 followed by a successful growth of the co-cultured biofilm in a 3D Alvatex platform.
AB - A study was performed to isolate and evaluate potential indigenous probiotic strains in Ghana. A total of 99 strains were isolated from human breast milk (n = 29) and fecal samples (n = 70), which were identified and characterized using methods ranging from plating growth tests and presumptive analysis at species level using MALDI-TOF MS, prior to validation through 16S rRNA gene sequence analysis. Gastrointestinal tolerance and ability to form biofilms in vitro were determined. Results indicated that out of 99 isolates, 25 were Gram-positive, catalase-negative rods. More than one-third was identified as Lactobacillus fermentum. Others were identified to be Lactobacillus (plantarum, rhamnosus, salivarius, reuteri), Enterococcus faecium, Weissella spp. and Pediococcus spp. Among the 25 isolates, 9 had activity against both Gram-negative and Gram-positive pathogens including reference or local clinical isolates of Pseudomonas aeruginosa, Salmonella Typhi, Staphylococcus aureus and Escherichia coli. Almost all isolates were effective against P. aeruginosa, E. coli and S. Typhi. Thirteen isolates did not show activity towards S. aureus. The isolates were more effective against local pathogens tested than nonindigenous pathogens. The data showed survival of all studied isolate at pH 2, 3 and 6 followed by a successful growth of the co-cultured biofilm in a 3D Alvatex platform.
KW - Indigenous strains
KW - Lactic acid bacteria (LAB)
KW - Lactobacillus
KW - Microbiome
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=85090194475&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2020.109895
DO - 10.1016/j.lwt.2020.109895
M3 - Article
AN - SCOPUS:85090194475
SN - 0023-6438
VL - 133
JO - LWT
JF - LWT
M1 - 109895
ER -