@inbook{42f1b221ca064a8494536c56d9a6b782,
title = "Primary Isolation of Mycobacterium ulcerans",
abstract = "Primary isolation of Mycobacterium ulcerans is the separation and growth of the bacterium from a mixed population either in clinical specimen or environmental specimen in pure cultures. It is a crucial activity as it can be used to monitor antimicrobial treatment, surveillance for antimicrobial resistance, and molecular epidemiology studies toward understanding pathogen ecology and transmission as well as pathogen biology. The process involves removal of unwanted fast-growing bacteria using 5% oxalic acid, inoculation on Lowenstein-Jensen medium supplemented with glycerol, and incubation at temperatures between 30 °C and 33 °C.",
keywords = "Clinical and environmental specimen, Decontamination, Mycobacterium ulcerans",
author = "Dorothy Yeboah-Manu and Adwoa Asante-Poku and {Yaw Aboagye}, Sammy",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1779-3_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--28",
booktitle = "Methods in Molecular Biology",
}