TY - JOUR
T1 - Plasmodium falciparum
T2 - Differing effects of non-esterified fatty acids and phospholipids on intraerythrocytic growth in serum-free medium
AU - Asahi, Hiroko
AU - Izumiyama, Shinji
AU - Tolba, Mohammed Essa Marghany
AU - Kwansa-Bentum, Bethel
PY - 2011/3
Y1 - 2011/3
N2 - Different combinations of non-esterified fatty acids (NEFA) had variable effects on intraerythrocytic growth of Plasmodium falciparum. All stages of the parasite cultured in medium supplemented with cis-9-octadecenoic acid (C18:1-cis-9), hexadecanoic acid (C16:0), phospholipids (Pld) and bovine albumin free of NEFA were similar to those grown in complete growth medium. Three typical growth patterns indicating suppressed schizogony (SS), suppressed formation of merozoites (SMF), and inhibited invasion of merozoites (IMI) resulted from culture in other combinations of lipids. Unsaturated or saturated NEFA with longer or shorter carbon chains than C18:1-cis-9 or C16:0, higher degree of unsaturation, and trans-forms mainly resulted in SS and SMF effects. However, IMI or partial IMI was observed with tetradecanoic acid or octadecanoic acid enriched with C18:1-cis-9, and cis-9-hexadecenoic acid plus C16:0. Isoforms of C18:1-cis-9 also mainly resulted in partial IMI. SMF also occurred with C18:1-cis-9 plus C16:0 in the absence of Pld. Thus different NEFA exerted distinct roles in erythrocytic growth of the parasite by sustaining development at different stages.
AB - Different combinations of non-esterified fatty acids (NEFA) had variable effects on intraerythrocytic growth of Plasmodium falciparum. All stages of the parasite cultured in medium supplemented with cis-9-octadecenoic acid (C18:1-cis-9), hexadecanoic acid (C16:0), phospholipids (Pld) and bovine albumin free of NEFA were similar to those grown in complete growth medium. Three typical growth patterns indicating suppressed schizogony (SS), suppressed formation of merozoites (SMF), and inhibited invasion of merozoites (IMI) resulted from culture in other combinations of lipids. Unsaturated or saturated NEFA with longer or shorter carbon chains than C18:1-cis-9 or C16:0, higher degree of unsaturation, and trans-forms mainly resulted in SS and SMF effects. However, IMI or partial IMI was observed with tetradecanoic acid or octadecanoic acid enriched with C18:1-cis-9, and cis-9-hexadecenoic acid plus C16:0. Isoforms of C18:1-cis-9 also mainly resulted in partial IMI. SMF also occurred with C18:1-cis-9 plus C16:0 in the absence of Pld. Thus different NEFA exerted distinct roles in erythrocytic growth of the parasite by sustaining development at different stages.
KW - Intraerythrocytic growth
KW - Non-esterified fatty acid
KW - Phospholipids
KW - Plasmodium falciparum
KW - Serum-free medium
UR - http://www.scopus.com/inward/record.url?scp=79951722526&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2010.11.001
DO - 10.1016/j.exppara.2010.11.001
M3 - Article
C2 - 21095186
AN - SCOPUS:79951722526
SN - 0014-4894
VL - 127
SP - 708
EP - 713
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -