Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis

Patrick K. Arthur, Maike Claussen, Susanne Koch, Katsiaryna Tarbashevich, Olaf Jahn, Tomas Pieler

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.

Original languageEnglish
Pages (from-to)19982-19992
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number30
DOIs
Publication statusPublished - 24 Jul 2009
Externally publishedYes

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