TY - JOUR
T1 - Participation of Xenopus Elr-type proteins in vegetal mRNA localization during oogenesis
AU - Arthur, Patrick K.
AU - Claussen, Maike
AU - Koch, Susanne
AU - Tarbashevich, Katsiaryna
AU - Jahn, Olaf
AU - Pieler, Tomas
PY - 2009/7/24
Y1 - 2009/7/24
N2 - Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.
AB - Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.
UR - http://www.scopus.com/inward/record.url?scp=67749118321&partnerID=8YFLogxK
U2 - 10.1074/jbc.M109.009928
DO - 10.1074/jbc.M109.009928
M3 - Article
C2 - 19458392
AN - SCOPUS:67749118321
SN - 0021-9258
VL - 284
SP - 19982
EP - 19992
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 30
ER -