Next-generation sequencing of a human-animal reassortant G6P[14] rotavirus A strain from a child hospitalized with diarrhoea

Susan Afua Damanka, Francis Ekow Dennis, Belinda Larteley Lartey, Kofi Mensah Nyarko, Chantal Ama Agbemabiese, George Enyimah Armah

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We previously reported the VP4 and the VP7 genotypes of the first G6P[14] rotavirus strain (RVA/Human-wt/GHA/M0084/2010/G6P[14]) from the stool of an infant with diarrhoea in Ghana. In the current study, we obtained the complete genome sequences using Illumina MiSeq next-generation sequencing to enable us to determine the host species origin of the genes by phylogenetic analysis. The genotype constellation was G6-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3. Phylogenetic analysis showed that M0084 was a reassortant strain from RVAs of both artiodactyl and human host species origin. The level of sequence identity of the individual genes of M0084 to other sequences in the GenBank ranged from 95.2 to 99.5%; however, there was no single strain from the GenBank database with a complete genome sequence that was highly similar to that of M0084. To help trace the source of such unique gene pools being introduced into human RVAs, it will be useful to examine RVA sequences from potential reservoirs such as sheep and goats, which are common domestic animals in this locality.

Original languageEnglish
Pages (from-to)1003-1005
Number of pages3
JournalArchives of Virology
Volume165
Issue number4
DOIs
Publication statusPublished - 1 Apr 2020
Externally publishedYes

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