TY - JOUR
T1 - Native SAG1 in Toxoplasma gondii lysates is superior to recombinant SAG1 for serodiagnosis of T. gondii infections in chickens
AU - Appiah-Kwarteng, Cornelia
AU - Saito, Taizo
AU - Toda, Natsuki
AU - Kitoh, Katsuya
AU - Nishikawa, Yoshibumi
AU - Adenyo, Christopher
AU - Kayang, Boniface
AU - Owusu, Ebenezer Oduro
AU - Ohya, Kenji
AU - Inoue-Murayama, Miho
AU - Kawahara, Fumiya
AU - Nagamune, Kisaburo
AU - Takashima, Yasuhiro
N1 - Publisher Copyright:
© 2019
PY - 2019/4
Y1 - 2019/4
N2 - Toxoplasma gondii can infect almost all mammals and birds, including chickens. The aim of this study was to identify an appropriate immunogenic antigen for serodiagnosis of T. gondii infections in chickens. We examined serum samples from chickens that were intravenously or intraperitoneally infected with 10 6 –10 8 tachyzoites of T. gondii strains PLK, RH, CTG, ME49 or TgCatJpGi1/TaJ using enzyme-linked immunosorbent assays (ELISAs), latex agglutination tests (LATs) and western blotting. Regardless of parasite strain or infection dose and route, the commercial LAT was positive for almost all sera collected 1 week post-infection. However, at 2 weeks post-infection, LATs were negative in the same birds. ELISAs using the Escherichia coli-produced recombinant T. gondii antigens SAG1 and GRA7 showed strong signals at 1–2 weeks post infection, but thereafter diminished for the majority of serum samples. In contrast, western blotting against crude tachyzoite antigens showed a persistent band up to 4 weeks post-infection. Sera from these chickens reacted much more strongly with SAG1 from crude tachyzoite antigens than with recombinant SAG1. Even in experimentally-infected birds whose parasite burdens in tissue were undetectable, sera still reacted with native SAG1. We tested sera from free-range chickens on a small farm in Ghana, Africa, using western blotting and found that the serum of one bird reacted with a single band of approximately 27 kDa, the putative molecular weight of SAG1. Thus we conclude that native SAG1, but not E. coli-produced recombinant SAG1, is suitable for serodiagnosis of T. gondii infections in chickens.
AB - Toxoplasma gondii can infect almost all mammals and birds, including chickens. The aim of this study was to identify an appropriate immunogenic antigen for serodiagnosis of T. gondii infections in chickens. We examined serum samples from chickens that were intravenously or intraperitoneally infected with 10 6 –10 8 tachyzoites of T. gondii strains PLK, RH, CTG, ME49 or TgCatJpGi1/TaJ using enzyme-linked immunosorbent assays (ELISAs), latex agglutination tests (LATs) and western blotting. Regardless of parasite strain or infection dose and route, the commercial LAT was positive for almost all sera collected 1 week post-infection. However, at 2 weeks post-infection, LATs were negative in the same birds. ELISAs using the Escherichia coli-produced recombinant T. gondii antigens SAG1 and GRA7 showed strong signals at 1–2 weeks post infection, but thereafter diminished for the majority of serum samples. In contrast, western blotting against crude tachyzoite antigens showed a persistent band up to 4 weeks post-infection. Sera from these chickens reacted much more strongly with SAG1 from crude tachyzoite antigens than with recombinant SAG1. Even in experimentally-infected birds whose parasite burdens in tissue were undetectable, sera still reacted with native SAG1. We tested sera from free-range chickens on a small farm in Ghana, Africa, using western blotting and found that the serum of one bird reacted with a single band of approximately 27 kDa, the putative molecular weight of SAG1. Thus we conclude that native SAG1, but not E. coli-produced recombinant SAG1, is suitable for serodiagnosis of T. gondii infections in chickens.
KW - Diagnostic
KW - Ghanaian field samples
KW - Immunogenicity
UR - http://www.scopus.com/inward/record.url?scp=85059672841&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2019.01.001
DO - 10.1016/j.parint.2019.01.001
M3 - Article
C2 - 30630114
AN - SCOPUS:85059672841
SN - 1383-5769
VL - 69
SP - 114
EP - 120
JO - Parasitology International
JF - Parasitology International
ER -