TY - JOUR
T1 - Molecular detection of dengue virus from febrile patients in Ghana
AU - Bonney, Joseph Humphrey Kofi
AU - Pratt, Deborah
AU - Ofori, Magdalene
AU - Hayashi, Takaya
AU - Abankwa, Abigail
AU - Awuku-Larbi, Yaw
AU - Kumordjie, Selassie
AU - Agbodzi, Bright
AU - Salisu, Musah
AU - Mante, Ama Amankwa Ofosua
AU - Bour, Stella
AU - Eshun, Miriam
AU - Amaning, Juliana Naa Dedei Acquah
AU - Ketorwoley, Prince
AU - Enimil, Nancy
AU - Koomson, Joel
AU - Stephens, Gertrude
AU - Asiedu-Bekoe, Franklin
AU - Laryea, Dennis
AU - Dadzie, Samuel
AU - Suzuki, Toshihiko
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Background: Viral hemorrhagic fevers (VHFs) belong to a group of viral infectious diseases that interfere with the blood’s clotting mechanism. VHF has a wide host range, including bats, rodents, or arthropods such as mosquitoes and ticks. Most VHFs emerge suddenly as outbreaks, making it difficult to predict occurrence. To be responsive to such outbreaks, the Noguchi Memorial Institute for Medical Research (NMIMR) provides high-end molecular and genomic diagnostics capability for surveillance of suspected VHFs in samples collected from health facilities across the country. Methods: Between January 2022 and December 2023, cross-sectional surveillance for viruses was conducted in patients with suspected VHF. During the period, 2586 serum or plasma samples were collected and transported under a cold chain to the NMIMR for testing. The samples were analyzed for potential VHF viruses including yellow fever, Ebola/Marburg, Lassa fever, and Dengue viruses using Real-Time Polymerase Chain Reaction Assay. Dengue positives were serotyped using the protocol of Johnson W.B et al.,2005. Whole genome sequencing was conducted using Illumina Next Generation Sequencing Technology. Using IQ-TREE, a maximum likelihood phylogenetic analysis was carried out. Results: Dengue virus (DENV) was detected in eight patient samples that subtyped to serotypes 1, 2, and 3. All dengue fever cases were resident in the Greater Accra region. The detection of serotype one increases the possibility of multiple infections in individuals and may have a worse or increased risk of severe dengue fever. Phylogenetic analysis revealed that the DENV-1 strain shared similarities to circulating strains in West Africa. Conclusion: Until the emergence of recent cases, the circulating subtype has been serotyped as Dengue one. There is therefore the need to intensify surveillance and also to control the mosquito vectors which can transmit these DENV in Ghana.
AB - Background: Viral hemorrhagic fevers (VHFs) belong to a group of viral infectious diseases that interfere with the blood’s clotting mechanism. VHF has a wide host range, including bats, rodents, or arthropods such as mosquitoes and ticks. Most VHFs emerge suddenly as outbreaks, making it difficult to predict occurrence. To be responsive to such outbreaks, the Noguchi Memorial Institute for Medical Research (NMIMR) provides high-end molecular and genomic diagnostics capability for surveillance of suspected VHFs in samples collected from health facilities across the country. Methods: Between January 2022 and December 2023, cross-sectional surveillance for viruses was conducted in patients with suspected VHF. During the period, 2586 serum or plasma samples were collected and transported under a cold chain to the NMIMR for testing. The samples were analyzed for potential VHF viruses including yellow fever, Ebola/Marburg, Lassa fever, and Dengue viruses using Real-Time Polymerase Chain Reaction Assay. Dengue positives were serotyped using the protocol of Johnson W.B et al.,2005. Whole genome sequencing was conducted using Illumina Next Generation Sequencing Technology. Using IQ-TREE, a maximum likelihood phylogenetic analysis was carried out. Results: Dengue virus (DENV) was detected in eight patient samples that subtyped to serotypes 1, 2, and 3. All dengue fever cases were resident in the Greater Accra region. The detection of serotype one increases the possibility of multiple infections in individuals and may have a worse or increased risk of severe dengue fever. Phylogenetic analysis revealed that the DENV-1 strain shared similarities to circulating strains in West Africa. Conclusion: Until the emergence of recent cases, the circulating subtype has been serotyped as Dengue one. There is therefore the need to intensify surveillance and also to control the mosquito vectors which can transmit these DENV in Ghana.
KW - Dengue fever
KW - Dengue serotypes
KW - Ghana
KW - NGS
KW - Phylogenetics
KW - Viral hemorrhagic fever
UR - http://www.scopus.com/inward/record.url?scp=85211342969&partnerID=8YFLogxK
U2 - 10.1186/s12879-024-10289-0
DO - 10.1186/s12879-024-10289-0
M3 - Article
C2 - 39627698
AN - SCOPUS:85211342969
SN - 1471-2334
VL - 24
JO - BMC Infectious Diseases
JF - BMC Infectious Diseases
IS - 1
M1 - 1382
ER -