Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Uri S. Markakpo; Kwabena M. Bosompem; Mawuli Dzodzomenyo; Anthony Danso-Appiah; Edward E. Essuman; William K. Anyan; Mitsuko Suzuki; Judith K. Stephens; Isaac Anim-Baidoo; Richard H. Asmah; Michael F. Ofori; Parnor Madjitey; Jonas B. Danquah; Naa Adjeley Frempong; Kofi D. Kwofie; Michael Amoa-Bosompem; David Sullivan; Julius N. Fobil; Isabella A. Quakyi

doi:10.1111/tmi.12744

Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Uri S. Markakpo, Kwabena M. Bosompem, Mawuli Dzodzomenyo, Anthony Danso-Appiah, Edward E. Essuman, William K. Anyan, Mitsuko Suzuki, Judith K. Stephens, Isaac Anim-Baidoo, Richard H. Asmah, Michael F. Ofori, Parnor Madjitey, Jonas B. Danquah, Naa Adjeley Frempong, Kofi D. Kwofie, Michael Amoa-Bosompem, David Sullivan, Julius N. Fobil, Isabella A. Quakyi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

Original language	English
Pages (from-to)	1263-1271
Number of pages	9
Journal	Tropical Medicine and International Health
Volume	21
Issue number	10
DOIs	https://doi.org/10.1111/tmi.12744
Publication status	Published - 1 Oct 2016

Keywords

Plasmodium
dipstick antigen monoclonal antibody
malaria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Markakpo, U. S., Bosompem, K. M., Dzodzomenyo, M., Danso-Appiah, A., Essuman, E. E., Anyan, W. K., Suzuki, M., Stephens, J. K., Anim-Baidoo, I., Asmah, R. H., Ofori, M. F., Madjitey, P., Danquah, J. B., Frempong, N. A., Kwofie, K. D., Amoa-Bosompem, M., Sullivan, D., Fobil, J. N., & Quakyi, I. A. (2016). Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria. Tropical Medicine and International Health, 21(10), 1263-1271. https://doi.org/10.1111/tmi.12744

@article{0941c68ae99342fb8c738e3f564bb619,

title = "Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria",

abstract = "Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.",

keywords = "Plasmodium, dipstick antigen monoclonal antibody, malaria",

author = "Markakpo, {Uri S.} and Bosompem, {Kwabena M.} and Mawuli Dzodzomenyo and Anthony Danso-Appiah and Essuman, {Edward E.} and Anyan, {William K.} and Mitsuko Suzuki and Stephens, {Judith K.} and Isaac Anim-Baidoo and Asmah, {Richard H.} and Ofori, {Michael F.} and Parnor Madjitey and Danquah, {Jonas B.} and Frempong, {Naa Adjeley} and Kwofie, {Kofi D.} and Michael Amoa-Bosompem and David Sullivan and Fobil, {Julius N.} and Quakyi, {Isabella A.}",

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year = "2016",

month = oct,

day = "1",

doi = "10.1111/tmi.12744",

language = "English",

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Markakpo, US, Bosompem, KM, Dzodzomenyo, M, Danso-Appiah, A, Essuman, EE, Anyan, WK, Suzuki, M, Stephens, JK, Anim-Baidoo, I, Asmah, RH, Ofori, MF, Madjitey, P, Danquah, JB, Frempong, NA, Kwofie, KD, Amoa-Bosompem, M, Sullivan, D, Fobil, JN & Quakyi, IA 2016, 'Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria', Tropical Medicine and International Health, vol. 21, no. 10, pp. 1263-1271. https://doi.org/10.1111/tmi.12744

TY - JOUR

T1 - Minimising invasiveness in diagnostics

T2 - developing a rapid urine-based monoclonal antibody dipstick test for malaria

AU - Markakpo, Uri S.

AU - Bosompem, Kwabena M.

AU - Dzodzomenyo, Mawuli

AU - Danso-Appiah, Anthony

AU - Essuman, Edward E.

AU - Anyan, William K.

AU - Suzuki, Mitsuko

AU - Stephens, Judith K.

AU - Anim-Baidoo, Isaac

AU - Asmah, Richard H.

AU - Ofori, Michael F.

AU - Madjitey, Parnor

AU - Danquah, Jonas B.

AU - Frempong, Naa Adjeley

AU - Kwofie, Kofi D.

AU - Amoa-Bosompem, Michael

AU - Sullivan, David

AU - Fobil, Julius N.

AU - Quakyi, Isabella A.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

AB - Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

KW - Plasmodium

KW - dipstick antigen monoclonal antibody

KW - malaria

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U2 - 10.1111/tmi.12744

DO - 10.1111/tmi.12744

M3 - Article

C2 - 27546068

AN - SCOPUS:84991086157

SN - 1360-2276

VL - 21

SP - 1263

EP - 1271

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 10

ER -

Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Abstract

Keywords

UN SDGs

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