TY - JOUR
T1 - Mechanically produced schistosomula as a higher-throughput tools for phenotypic pre-screening in drug sensitivity assays
T2 - Current research and future trends
AU - Tekwu, Emmanuel Mouafo
AU - Anyan, William Kofi
AU - Boamah, Daniel
AU - Baffour-Awuah, Kofi Owusu
AU - Tekwu, Stephanie Keyetat
AU - Beng, Veronique Penlap
AU - Nyarko, Alexander Kwadwo
AU - Bosompem, Kwabena Mante
N1 - Publisher Copyright:
© The Author(s). 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
PY - 2016
Y1 - 2016
N2 - It is crucial to develop new antischistosomal drugs since there is no vaccine and the whole world is relying on only a single drug for the treatment of schistosomiasis. One of the obstacles to the development of drugs is the absence of the high throughput objective screening methods to assess drug compounds efficacy. Thus for identification of new drug compounds candidates, fast and accurate in vitro assays are unavoidable and more research efforts in the field of drug discovery can target schistosomula. This review presents a substantial overview of the present state of in vitro drug sensitivity assays developed so far for the determination of anti-schistosomula activity of drug compounds, natural products and derivatives using newly transformed schistosomula (NTS). It highlights some of the challenges involved in in vitro compound screening using NTS and the way forward.
AB - It is crucial to develop new antischistosomal drugs since there is no vaccine and the whole world is relying on only a single drug for the treatment of schistosomiasis. One of the obstacles to the development of drugs is the absence of the high throughput objective screening methods to assess drug compounds efficacy. Thus for identification of new drug compounds candidates, fast and accurate in vitro assays are unavoidable and more research efforts in the field of drug discovery can target schistosomula. This review presents a substantial overview of the present state of in vitro drug sensitivity assays developed so far for the determination of anti-schistosomula activity of drug compounds, natural products and derivatives using newly transformed schistosomula (NTS). It highlights some of the challenges involved in in vitro compound screening using NTS and the way forward.
KW - Drug discovery
KW - In vitro drug sensitivity
KW - Mechanical transformation
KW - Newly Transformed Schistosomula
KW - Schistosomiasis
UR - http://www.scopus.com/inward/record.url?scp=85058432947&partnerID=8YFLogxK
U2 - 10.1186/s40364-016-0075-2
DO - 10.1186/s40364-016-0075-2
M3 - Review article
AN - SCOPUS:85058432947
SN - 2050-7771
VL - 4
JO - Biomarker Research
JF - Biomarker Research
IS - 1
M1 - 21
ER -
