Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens

Dorothy Yeboah-Manu; Emelia Danso; Kobina Ampah; Adwoa Asante-Poku; Zuliehatu Nakobu; Gerd Pluschke

doi:10.1128/JCM.02279-10

Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens

Dorothy Yeboah-Manu
, Emelia Danso
, Kobina Ampah
, Adwoa Asante-Poku
, Zuliehatu Nakobu
, Gerd Pluschke

Department of Bacteriology

University of Ghana
Swiss Tropical and Public Health Institute Swiss TPH
University of Basel

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

For cultivation of Mycobacterium ulcerans from clinical specimens, we optimized the release of bacteria from swabs, as well as decontamination and cultivation on supplemented medium. Nevertheless, the proportions of positive cultures, 41.7% (5/12) for fine-needle-aspiration (FNA) samples and 43.8% (49/112) for swab samples, were lower than those we have previously observed for excised tissue specimens.

Original language	English
Pages (from-to)	1997-1999
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	5
DOIs	https://doi.org/10.1128/JCM.02279-10
Publication status	Published - May 2011

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abstract = "For cultivation of Mycobacterium ulcerans from clinical specimens, we optimized the release of bacteria from swabs, as well as decontamination and cultivation on supplemented medium. Nevertheless, the proportions of positive cultures, 41.7\% (5/12) for fine-needle-aspiration (FNA) samples and 43.8\% (49/112) for swab samples, were lower than those we have previously observed for excised tissue specimens.",

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AU - Nakobu, Zuliehatu

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AB - For cultivation of Mycobacterium ulcerans from clinical specimens, we optimized the release of bacteria from swabs, as well as decontamination and cultivation on supplemented medium. Nevertheless, the proportions of positive cultures, 41.7% (5/12) for fine-needle-aspiration (FNA) samples and 43.8% (49/112) for swab samples, were lower than those we have previously observed for excised tissue specimens.

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Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens

Abstract

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