TY - JOUR
T1 - Integrative genome-wide analysis reveals the role of WIP proteins in inhibition of growth and development
AU - Roldan, Maria Victoria Gomez
AU - Izhaq, Farhaj
AU - Verdenaud, Marion
AU - Eleblu, John
AU - Haraghi, Aimen
AU - Sommard, Vivien
AU - Chambrier, Pierre
AU - Latrasse, David
AU - Jégu, Teddy
AU - Benhamed, Moussa
AU - Szécsi, Judit
AU - Bendahmane, Mohammed
AU - Boualem, Adnane
AU - Bendahmane, Abdelhafid
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - In cucurbits, CmWIP1 is a master gene controlling sex determination. To bring new insight in the function of CmWIP1, we investigated two Arabidopsis WIP transcription factors, AtWIP1/TT1 and AtWIP2/NTT. Using an inducible system we showed that WIPs are powerful inhibitor of growth and inducer of cell death. Using ChIP-seq and RNA-seq we revealed that most of the up-regulated genes bound by WIPs display a W-box motif, associated with stress signaling. In contrast, the down-regulated genes contain a GAGA motif, a known target of polycomb repressive complex. To validate the role of WIP proteins in inhibition of growth, we expressed AtWIP1/TT1 in carpel primordia and obtained male flowers, mimicking CmWIP1 function in melon. Using other promoters, we further demonstrated that WIPs can trigger growth arrest of both vegetative and reproductive organs. Our data supports an evolutionary conserved role of WIPs in recruiting gene networks controlling growth and adaptation to stress.
AB - In cucurbits, CmWIP1 is a master gene controlling sex determination. To bring new insight in the function of CmWIP1, we investigated two Arabidopsis WIP transcription factors, AtWIP1/TT1 and AtWIP2/NTT. Using an inducible system we showed that WIPs are powerful inhibitor of growth and inducer of cell death. Using ChIP-seq and RNA-seq we revealed that most of the up-regulated genes bound by WIPs display a W-box motif, associated with stress signaling. In contrast, the down-regulated genes contain a GAGA motif, a known target of polycomb repressive complex. To validate the role of WIP proteins in inhibition of growth, we expressed AtWIP1/TT1 in carpel primordia and obtained male flowers, mimicking CmWIP1 function in melon. Using other promoters, we further demonstrated that WIPs can trigger growth arrest of both vegetative and reproductive organs. Our data supports an evolutionary conserved role of WIPs in recruiting gene networks controlling growth and adaptation to stress.
UR - http://www.scopus.com/inward/record.url?scp=85084812958&partnerID=8YFLogxK
U2 - 10.1038/s42003-020-0969-2
DO - 10.1038/s42003-020-0969-2
M3 - Article
C2 - 32415243
AN - SCOPUS:85084812958
SN - 2399-3642
VL - 3
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 239
ER -