TY - JOUR
T1 - Indirect plant regeneration in Dioscorea rotundata and Dioscorea alata yam genotypes using different explants
AU - Azu, Elaine
AU - Elegba, Wilfred
AU - Asare, Abigail Tweneboah
AU - Asare, Kwame
AU - Akama, Christian
AU - Otu, Solomon Ayeboafo
AU - Azure, Samuel
AU - Narkwa, Nanabanyin Kweku Nifa
AU - Owusu Ayeh, Kwadwo
AU - Danso, Kenneth
N1 - Publisher Copyright:
© 2024 Ghana Atomic Energy Commission, Accra, Ghana.
PY - 2024
Y1 - 2024
N2 - Yam (Dioscorea sp.) is an important staple crop for millions of smallholder farmers in West Africa. In this study, we aimed to develop an efficient indirect plant regeneration system for three important yam genotypes “Kukrupa,” “Mankrong Pona” and “Matches.” The potential of leaf primordia, petioles and axillary bud explants for callus induction on Murashige and Skoog (MS) medium supplemented with different concentrations (0, 3, 5, 7 and 9 mg/l) of 2,4-dicholorophenoxy acetic acid (2,4-D) or picloram were tested. Callus induction was influenced by auxin concentration as well as explant. The highest callus induction was achieved using leaf primordia (“Kukrupa” and “Mankrong Pona”) explants on 3 mg/l picloram or axillary buds (“Matches”) on 5 mg/l picloram. Transfer of callus to 6-benzylaminoapurine (BAP) amended medium resulted in the formation of at least two shoots per callus clump. Plantlets were obtained on basic yam medium supplemented with 0.2 mg/l 1-naphthalene acetic acid (NAA), 3 mg/l kinetin and 0.5 mg/l BAP after four weeks. Post acclimatization plantlet survival was high (75 − 82.5%). The indirect plant regeneration protocol reported through this study helps in the improvement of important yam genotypes through tissue culture-oriented genome editing, in vitro mutagenesis and also for its mass propagation.
AB - Yam (Dioscorea sp.) is an important staple crop for millions of smallholder farmers in West Africa. In this study, we aimed to develop an efficient indirect plant regeneration system for three important yam genotypes “Kukrupa,” “Mankrong Pona” and “Matches.” The potential of leaf primordia, petioles and axillary bud explants for callus induction on Murashige and Skoog (MS) medium supplemented with different concentrations (0, 3, 5, 7 and 9 mg/l) of 2,4-dicholorophenoxy acetic acid (2,4-D) or picloram were tested. Callus induction was influenced by auxin concentration as well as explant. The highest callus induction was achieved using leaf primordia (“Kukrupa” and “Mankrong Pona”) explants on 3 mg/l picloram or axillary buds (“Matches”) on 5 mg/l picloram. Transfer of callus to 6-benzylaminoapurine (BAP) amended medium resulted in the formation of at least two shoots per callus clump. Plantlets were obtained on basic yam medium supplemented with 0.2 mg/l 1-naphthalene acetic acid (NAA), 3 mg/l kinetin and 0.5 mg/l BAP after four weeks. Post acclimatization plantlet survival was high (75 − 82.5%). The indirect plant regeneration protocol reported through this study helps in the improvement of important yam genotypes through tissue culture-oriented genome editing, in vitro mutagenesis and also for its mass propagation.
KW - 2,4-dichlorophenoxyacetic acid
KW - Callus induction
KW - explants
KW - leaf primordia
KW - picloram
KW - plantlet regeneration
KW - smallholder farmers
UR - http://www.scopus.com/inward/record.url?scp=85196625371&partnerID=8YFLogxK
U2 - 10.1080/11263504.2024.2360465
DO - 10.1080/11263504.2024.2360465
M3 - Article
AN - SCOPUS:85196625371
SN - 1126-3504
VL - 158
SP - 788
EP - 795
JO - Plant Biosystems
JF - Plant Biosystems
IS - 4
ER -