TY - JOUR
T1 - In Vitro Activity of Selected West African Medicinal Plants against Mycobacterium ulcerans Disease
AU - Fokou, Patrick Valere Tsouh
AU - Kissi-Twum, Abena Adomah
AU - Yeboah-Manu, Dorothy
AU - Appiah-Opong, Regina
AU - Addo, Phyllis
AU - Yamthe, Lauve Rachel Tchokouaha
AU - Mfopa, Alvine Ngoutane
AU - Boyom, Fabrice Fekam
AU - Nyarko, Alexander Kwadwo
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2016/4
Y1 - 2016/4
N2 - Buruli ulcer (BU) is the third most prevalent mycobacteriosis, after tuberculosis and leprosy. The currently recommended combination of rifampicin-streptomycin suffers from side effects and poor compliance, which leads to reliance on local herbal remedies. The objective of this study was to investigate the antimycobacterial properties and toxicity of selected medicinal plants. Sixty-five extracts from 27 plant species were screened against Mycobacterium ulcerans and Mycobacterium smegmatis, using the Resazurin Microtiter Assay (REMA). The cytotoxicity of promising extracts was assayed on normal Chang liver cells by an MTT assay. Twenty five extracts showed activity with minimal inhibitory concentration (MIC) values ranging from 16 μg/mL to 250 μg/mL against M. smegmatis, while 17 showed activity against M. ulcerans with MIC values ranging from 125 μg/mL to 250 μg/mL. In most of the cases, plant extracts with antimycobacterial activity showed no cytotoxicity on normal human liver cells. Exception were Carica papaya, Cleistopholis patens, and Polyalthia suaveolens with 50% cell cytotoxic concentrations (CC50 ) ranging from 3.8 to 223 μg/mL. These preliminary results support the use of some West African plants in the treatment of Buruli ulcer. Meanwhile, further studies are required to isolate and characterize the active ingredients in the extracts.
AB - Buruli ulcer (BU) is the third most prevalent mycobacteriosis, after tuberculosis and leprosy. The currently recommended combination of rifampicin-streptomycin suffers from side effects and poor compliance, which leads to reliance on local herbal remedies. The objective of this study was to investigate the antimycobacterial properties and toxicity of selected medicinal plants. Sixty-five extracts from 27 plant species were screened against Mycobacterium ulcerans and Mycobacterium smegmatis, using the Resazurin Microtiter Assay (REMA). The cytotoxicity of promising extracts was assayed on normal Chang liver cells by an MTT assay. Twenty five extracts showed activity with minimal inhibitory concentration (MIC) values ranging from 16 μg/mL to 250 μg/mL against M. smegmatis, while 17 showed activity against M. ulcerans with MIC values ranging from 125 μg/mL to 250 μg/mL. In most of the cases, plant extracts with antimycobacterial activity showed no cytotoxicity on normal human liver cells. Exception were Carica papaya, Cleistopholis patens, and Polyalthia suaveolens with 50% cell cytotoxic concentrations (CC50 ) ranging from 3.8 to 223 μg/mL. These preliminary results support the use of some West African plants in the treatment of Buruli ulcer. Meanwhile, further studies are required to isolate and characterize the active ingredients in the extracts.
KW - Buruli ulcer
KW - Cytotoxicity
KW - Medicinal plants
KW - Mycobacterium ulcerans
UR - http://www.scopus.com/inward/record.url?scp=84966359608&partnerID=8YFLogxK
U2 - 10.3390/molecules21040445
DO - 10.3390/molecules21040445
M3 - Article
C2 - 27089314
AN - SCOPUS:84966359608
SN - 1420-3049
VL - 21
JO - Molecules
JF - Molecules
IS - 4
M1 - 445
ER -