Immune response in mice immunized with chimeric H1 antigens

Erasmus Nikoi Kotey; William Kwabena Ampofo; Rebecca Daines; Jean Remy Sadeyen; Munir Iqbal; Osbourne Quaye

doi:10.3390/VACCINES9101182

Immune response in mice immunized with chimeric H1 antigens

Erasmus Nikoi Kotey
, William Kwabena Ampofo
, Rebecca Daines
, Jean Remy Sadeyen
, Munir Iqbal
, Osbourne Quaye

Department of Biochemistry, Cell And Molecular Biology

University of Ghana
The Pirbright Institute
Royal Veterinary College University of London

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds potential for further development of a universal influenza vaccine.

Original language	English
Article number	1182
Journal	Vaccines
Volume	9
Issue number	10
DOIs	https://doi.org/10.3390/VACCINES9101182
Publication status	Published - Oct 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Broad-reactive antibodies
Chimeric haemagglutinin
Cross-reactivity
Prophylactic
Seasonal influenza
Therapeutic
Universal influenza vaccine

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10.3390/VACCINES9101182

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title = "Immune response in mice immunized with chimeric H1 antigens",

abstract = "Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus{\textquoteright} HA and holds potential for further development of a universal influenza vaccine.",

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doi = "10.3390/VACCINES9101182",

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T1 - Immune response in mice immunized with chimeric H1 antigens

AU - Kotey, Erasmus Nikoi

AU - Ampofo, William Kwabena

AU - Daines, Rebecca

AU - Sadeyen, Jean Remy

AU - Iqbal, Munir

AU - Quaye, Osbourne

PY - 2021/10

Y1 - 2021/10

N2 - Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds potential for further development of a universal influenza vaccine.

AB - Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds potential for further development of a universal influenza vaccine.

KW - Broad-reactive antibodies

KW - Chimeric haemagglutinin

KW - Cross-reactivity

KW - Prophylactic

KW - Seasonal influenza

KW - Therapeutic

KW - Universal influenza vaccine

UR - https://www.scopus.com/pages/publications/85121128747

U2 - 10.3390/VACCINES9101182

DO - 10.3390/VACCINES9101182

M3 - Article

AN - SCOPUS:85121128747

SN - 2076-393X

VL - 9

JO - Vaccines

JF - Vaccines

IS - 10

M1 - 1182

ER -

Immune response in mice immunized with chimeric H1 antigens

Abstract

UN SDGs

Keywords

Access to Document

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