Identification of hepatitis B virus genotype A/E recombinants in Ghana

Ceejay L. Boyce; Stephaney Willis; Timothy N.A. Archampong; Margaret Lartey; Kwamena W. Sagoe; Adjoa Obo-Akwa; Ernest Kenu; Awewura Kwara; Jason T. Blackard

doi:10.1007/s11262-019-01690-y

Identification of hepatitis B virus genotype A/E recombinants in Ghana

Ceejay L. Boyce
, Stephaney Willis
, Timothy N.A. Archampong
, Margaret Lartey
, Kwamena W. Sagoe
, Adjoa Obo-Akwa
, Ernest Kenu
, Awewura Kwara
, Jason T. Blackard

Department of Medical Microbiology

University of Cincinnati
University of Ghana
Korle Bu Teaching Hospital
University of Florida

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) exhibits a high degree of heterogeneity with at least 10 genotypes (A–J) identified to date. Intergenotypic recombination is relatively common. Previously, we investigated HBV drug resistance in HIV/HBV co-infected individuals in Ghana. After identifying multiple circulating genotypes and a novel D/E recombinant, we sought to determine if additional individuals were also infected with recombinant HBV. Partial genome sequences from three individuals were initially identified as genotype A4. Full-length HBV genomes were obtained using rolling circle amplification followed by PCR and shown to cluster with known A/E recombinant viruses. Similar recombination breakpoints were observed in these three individuals suggesting local spread of this novel recombinant HBV in Ghana.

Original language	English
Pages (from-to)	707-712
Number of pages	6
Journal	Virus Genes
Volume	55
Issue number	5
DOIs	https://doi.org/10.1007/s11262-019-01690-y
Publication status	Published - 1 Oct 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Africa
Genotype
Ghana
Hepatitis B virus
Recombinant/recombination

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10.1007/s11262-019-01690-y

Cite this

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title = "Identification of hepatitis B virus genotype A/E recombinants in Ghana",

abstract = "Hepatitis B virus (HBV) exhibits a high degree of heterogeneity with at least 10 genotypes (A–J) identified to date. Intergenotypic recombination is relatively common. Previously, we investigated HBV drug resistance in HIV/HBV co-infected individuals in Ghana. After identifying multiple circulating genotypes and a novel D/E recombinant, we sought to determine if additional individuals were also infected with recombinant HBV. Partial genome sequences from three individuals were initially identified as genotype A4. Full-length HBV genomes were obtained using rolling circle amplification followed by PCR and shown to cluster with known A/E recombinant viruses. Similar recombination breakpoints were observed in these three individuals suggesting local spread of this novel recombinant HBV in Ghana.",

keywords = "Africa, Genotype, Ghana, Hepatitis B virus, Recombinant/recombination",

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doi = "10.1007/s11262-019-01690-y",

language = "English",

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AU - Boyce, Ceejay L.

AU - Willis, Stephaney

AU - Archampong, Timothy N.A.

AU - Lartey, Margaret

AU - Sagoe, Kwamena W.

AU - Obo-Akwa, Adjoa

AU - Kenu, Ernest

AU - Kwara, Awewura

AU - Blackard, Jason T.

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AB - Hepatitis B virus (HBV) exhibits a high degree of heterogeneity with at least 10 genotypes (A–J) identified to date. Intergenotypic recombination is relatively common. Previously, we investigated HBV drug resistance in HIV/HBV co-infected individuals in Ghana. After identifying multiple circulating genotypes and a novel D/E recombinant, we sought to determine if additional individuals were also infected with recombinant HBV. Partial genome sequences from three individuals were initially identified as genotype A4. Full-length HBV genomes were obtained using rolling circle amplification followed by PCR and shown to cluster with known A/E recombinant viruses. Similar recombination breakpoints were observed in these three individuals suggesting local spread of this novel recombinant HBV in Ghana.

KW - Africa

KW - Genotype

KW - Ghana

KW - Hepatitis B virus

KW - Recombinant/recombination

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DO - 10.1007/s11262-019-01690-y

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AN - SCOPUS:85069653806

SN - 0920-8569

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SP - 707

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JO - Virus Genes

JF - Virus Genes

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ER -

Identification of hepatitis B virus genotype A/E recombinants in Ghana

Abstract

UN SDGs

Keywords

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