Histomorphology of placentae of women with sickle cell disease during pregnancy - A case control study

Mohammed Mumuni, Kevin Kofi Adutwum-Ofosu, Benjamin Arko-Boham, Bismarck Afedo Hottor, Nii Koney Kwaku Koney, Kwame Adu-Bonsaffoh, Samuel Antwi Oppong, Peter Ofori Appiah, John Ahenkorah

Research output: Contribution to journalArticlepeer-review

Abstract

Background Sickle cell disease (SCD) is known to exert multifaceted effects on pregnancy, potentially influencing placental structure and function. Aim Our aim was to utilize stereology as a precise analytical tool to evaluate the histo-morphologic and functional changes in term placentae of women with SCD against those of non-SCD women. Method A case control study was conducted at the Korle-Bu Teaching Hospital’s labour unit and included 38 pregnant women, comprising 19 cases and 19 controls. Placenta samples were paired and matched with gestational age and taken at term (38 weeks + 2 weeks). Tissue sections were prepared, stained with hematoxylin and eosin, and volume densities of syncytial knots, foetal capillaries, syncytial denuded areas, and intervillous spaces estimated by stereological methods. Statistical analysis was performed to compare mean values between the SCD and control groups. Results Among the study participants with SCD, 13.16% (5) had sickle cell haemoglobin S (HbSS), 34.21% (13) had haemoglobin C (HbSC) and 2.63% (1) had β-thalassemia (HbS). On stereological assessment, there were statistically significant differences in mean volume densities of syncytial knots (p = < 0.0034), foetal capillaries (p = < 0.0001), syncytial denudations (p = < 0.0028), and intervillous space (p = < 0.0113) between term placentae of women with SCD and those without SCD. Conclusion SCD placentae may result in a substantial increase in syncytial knot formation, possibly because of hypermaturation of the chorionic villi, significant increase in foetal capillaries potentially due to the hypoxic nature of the SCD placentae, syncytial denuded areas as a result of alteration of the placental syncytium and reduced intervillous spaces which may be due to villous congestion. These findings suggest the need for heightened monitoring of placental function and fetal well-being in pregnancies complicated by SCD to reduce adverse perinatal outcomes.

Original languageEnglish
Article numbere0319011
JournalPLoS ONE
Volume20
Issue number2 February
DOIs
Publication statusPublished - Feb 2025

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