Abstract
Extremely low-frequency electromagnetic field (ELFEF) is a well-known mechanical stimulation that induces neural differentiation. It is potentially an effective treatment for neurodegenerative diseases. In a previous study, ferritin light chain was upregulated in ELFEF-exposed human bone marrow-derived mesenchymal stem cells (BM-MSCs). Ferritin light chain is a component of ferritin, a highly conserved iron-binding protein. In this study, to identify molecules associated with ferritin during neural differentiation of BM-MSCs, we performed reverse transcription polymerase chain reaction (RT-PCR), western blotting, and ATP analysis. Our data indicated that ELFEF triggers the upregulation of ferritin light chain (FLC) and ferritin heavy chain (FHC) in BM-MSCs. The elevated levels of FLC and FHC correlated positively with the differentiation of BM-MSCs into neural cells. Moreover ELFEF induced the activation of iron regulatory protein-1 (IRP-1) and cofilin, which are downstream targets of ferritin. These results suggest that ELFEF induces neural differentiation through activation of a ferritin-regulated mechanism.
Original language | English |
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Pages (from-to) | 55-59 |
Number of pages | 5 |
Journal | Cellular and Molecular Biology |
Volume | 61 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Keywords
- Bone marrow-derived mesenchymal stem cells (BM-MSCS)
- Cofilin
- Extremely low-frequency electromagnetic field (ElFEF)
- Ferritin
- Iron regulatory protein-1 (IRP-1)
- Neural differentiation