Ex Vivo Plasmodium malariae Culture Method for Antimalarial Drugs Screen in the Field

Laurent Dembele, Nouhoum Diallo, Fanta Sogore, Bintou Diarra, Fatoumata I. Ballo, Amadou Daou, Ousmaila Diakite, Yacouba Bare, Cheick Papa Oumar Sangare, Aboubecrin Sedhigh Haidara, Seidina A.S. Diakite, Amadou Niangaly, Mahamadou Diakite, Brice Campo, Gordon A. Awandare, Yaw Aniweh, Abdoulaye A. Djimde

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In vitro and ex vivo cultivation of Plasmodium (P) falciparum has facilitated active research into the malaria parasite toward the quest for basic knowledge and the discovery of effective drug treatments. Such a drug discovery program is currently difficult for P. malariae simply because of the absence of in vitro and ex vivo cultivation system for its asexual blood stages supporting antimalarial evaluation. Despite availability of artemisinin combination therapies effective on P. falciparum, P. malariae is being increasingly detected in malaria endemic countries. P. malariae is responsible for chronic infections and is associated with a high burden of anemia and morbidity. Here, we optimized and adapted ex vivo conditions under which P. malariae can be cultured and used for screening antimalarial drugs. Subsequently, this enabled us to test compounds such as artemether, chloroquine, lumefantrine, and quinine for ex vivo antimalarial activity against P. malariae.

Original languageEnglish
Pages (from-to)3025-3033
Number of pages9
JournalACS Infectious Diseases
Volume7
Issue number11
DOIs
Publication statusPublished - 12 Nov 2021

Keywords

  • Plasmodium malariae
  • drug discovery
  • ex vivo culture
  • malaria
  • nonfalciparum

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