TY - JOUR
T1 - Evaluation of serum metallothionein-1, selenium, zinc, and copper in Ghanaian type 2 diabetes mellitus patients
AU - Asare, George Awuku
AU - Osae, Shiloh
AU - Nortey, Ezekiel Nii Noye
AU - Yambire, Faisal King
AU - Amedonu, Elsie
AU - Doku, Derick
AU - Annan, Yvonne
PY - 2013/6
Y1 - 2013/6
N2 - Metabolic disturbances of trace elements may be implicated in the complications of type 2 diabetes mellitus (T2DM). The aim of the study was to determine the level of Zinc (Zn), Selenium (Se), Copper (Cu) and the metal binding protein Metallothionein-1 (MT-1) in T2DM. Fifty-five (55) T2DM subjects and 30 Controls (C) were studied for, Se, Zn, Cu and MT-1. Zn, Se and Cu were analyzed using Flame Atomic Absorption Spectroscopy. Mean FBG in the T2DM and C groups were 183 ± 5 mg/dl and 88 ± 5 mg/dl, respectively. Mean Se, Zn and Cu levels in the T2DM group were 204 ± 91 μg/l, 407 ± 117 μg/l and 1,337 ± 527 μg/l, respectively. The control group had Se, Zn and Cu levels of 123 ± 25 μg/l, 750 ± 190 μg/l and 989 ± 197 μg/l, respectively. While Zn levels in T2DM were half that of the C, Se levels were ≈ 2-fold. Se, Zn and Cu differences between the two groups were statistically significant (P = 0.000; P = 0.000, P = 0.000, respectively). The metabolic derailment of MT-1 in the T2DM group showed a wide variation with the T2DM having significantly lower MT-1 values (P = 0.000). A negative correlation was seen between Cu and Zn in the T2DM group (P = 0.022). A standardized canonical discriminant function was obtained as D = 0.823FBG-0.149MT-0.457Zn + 0.172Cu + 0.362Se with contributions of FBG > Zn > Se > Cu > MT-1. In conclusion, alterations in the levels of Zn, Se and Cu were observed in Ghanaian T2DM patients.
AB - Metabolic disturbances of trace elements may be implicated in the complications of type 2 diabetes mellitus (T2DM). The aim of the study was to determine the level of Zinc (Zn), Selenium (Se), Copper (Cu) and the metal binding protein Metallothionein-1 (MT-1) in T2DM. Fifty-five (55) T2DM subjects and 30 Controls (C) were studied for, Se, Zn, Cu and MT-1. Zn, Se and Cu were analyzed using Flame Atomic Absorption Spectroscopy. Mean FBG in the T2DM and C groups were 183 ± 5 mg/dl and 88 ± 5 mg/dl, respectively. Mean Se, Zn and Cu levels in the T2DM group were 204 ± 91 μg/l, 407 ± 117 μg/l and 1,337 ± 527 μg/l, respectively. The control group had Se, Zn and Cu levels of 123 ± 25 μg/l, 750 ± 190 μg/l and 989 ± 197 μg/l, respectively. While Zn levels in T2DM were half that of the C, Se levels were ≈ 2-fold. Se, Zn and Cu differences between the two groups were statistically significant (P = 0.000; P = 0.000, P = 0.000, respectively). The metabolic derailment of MT-1 in the T2DM group showed a wide variation with the T2DM having significantly lower MT-1 values (P = 0.000). A negative correlation was seen between Cu and Zn in the T2DM group (P = 0.022). A standardized canonical discriminant function was obtained as D = 0.823FBG-0.149MT-0.457Zn + 0.172Cu + 0.362Se with contributions of FBG > Zn > Se > Cu > MT-1. In conclusion, alterations in the levels of Zn, Se and Cu were observed in Ghanaian T2DM patients.
KW - Diabetes
KW - Ghanaian
KW - Oxidative stress
KW - Trace elements
UR - http://www.scopus.com/inward/record.url?scp=84880274712&partnerID=8YFLogxK
U2 - 10.1007/s13410-013-0111-9
DO - 10.1007/s13410-013-0111-9
M3 - Article
AN - SCOPUS:84880274712
SN - 0973-3930
VL - 33
SP - 86
EP - 95
JO - International Journal of Diabetes in Developing Countries
JF - International Journal of Diabetes in Developing Countries
IS - 2
ER -