TY - JOUR
T1 - Establishment of a quantitative and qualitative analysis and isolation method for tetracyclic iridoids from Morinda lucida Bentham leaves
AU - Ohta, Tomoe
AU - Tilkanont, Tanatorn
AU - Ayertey, Frederick
AU - Nakagawa, Mina
AU - Tung, Nguyen Huu
AU - Bolah, Peter
AU - Blagogee, Heron
AU - Appiah, Alfred Ampomah
AU - Ocloo, Augustine
AU - Ohashi, Mitsuko
AU - Tanoue, Kensuke
AU - Yamaguchi, Yasuchika
AU - Ohta, Nobuo
AU - Yamaoka, Shoji
AU - Iwanaga, Shiro
AU - Uto, Takuhiro
AU - Shoyama, Yukihiro
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/2/5
Y1 - 2019/2/5
N2 - A new high performance liquid chromatography (HPLC) method has been established for quantitative and qualitative analysis of three tetracyclic iridoids: ML-2-3 (1), molucidin (2), and ML-F52 (3), which are responsible for anti-trypanosomal and anti-leishmanial activities of Morinda lucida Bentham leaves. Separation of 1–3 from dried 80% aqueous (aq.) ethanol extract was achieved on a reversed-phase cholester column packed with cholesteryl-bonded silica using an acetonitrile-0.1% aq. formic acid mobile phase system. Ultraviolet-visible (UV-VIS) spectroscopy was employed for detection of compounds, and their contents were determined by measuring absorbance at 254 nm. Depending on the above system, several factors potentially affecting the concentration of tetracyclic iridoids were evaluated resulting in several variation on plant organs, seasonality, variation between individual trees, and branch positions within the trees. Moreover, we developed a simple, quick, and effective method for tetracyclic iridoid isolation from M. lucida leaves that consisted of extraction by sonication into 80% aq. ethanol, basic hydrolysis, acid neutralization, liquid-liquid extraction into an organic solvent, and reverse phase open column chromatography. Employing this method, we have succeeded to obtain 1 as a colorless crystal yielding of 0.23%, which was 28 times higher than that of previous isolation method. Setting up methodology in this paper may be important for future in vitro and in vivo studies of tetracyclic iridoids and moreover for their applications in new drug design and development.
AB - A new high performance liquid chromatography (HPLC) method has been established for quantitative and qualitative analysis of three tetracyclic iridoids: ML-2-3 (1), molucidin (2), and ML-F52 (3), which are responsible for anti-trypanosomal and anti-leishmanial activities of Morinda lucida Bentham leaves. Separation of 1–3 from dried 80% aqueous (aq.) ethanol extract was achieved on a reversed-phase cholester column packed with cholesteryl-bonded silica using an acetonitrile-0.1% aq. formic acid mobile phase system. Ultraviolet-visible (UV-VIS) spectroscopy was employed for detection of compounds, and their contents were determined by measuring absorbance at 254 nm. Depending on the above system, several factors potentially affecting the concentration of tetracyclic iridoids were evaluated resulting in several variation on plant organs, seasonality, variation between individual trees, and branch positions within the trees. Moreover, we developed a simple, quick, and effective method for tetracyclic iridoid isolation from M. lucida leaves that consisted of extraction by sonication into 80% aq. ethanol, basic hydrolysis, acid neutralization, liquid-liquid extraction into an organic solvent, and reverse phase open column chromatography. Employing this method, we have succeeded to obtain 1 as a colorless crystal yielding of 0.23%, which was 28 times higher than that of previous isolation method. Setting up methodology in this paper may be important for future in vitro and in vivo studies of tetracyclic iridoids and moreover for their applications in new drug design and development.
KW - Anti-leishmania
KW - Anti-trypanosoma
KW - Isolation
KW - Morinda lucida
KW - Quantitative analysis
KW - Tetracyclic iridoids
UR - http://www.scopus.com/inward/record.url?scp=85056883953&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2018.10.044
DO - 10.1016/j.jpba.2018.10.044
M3 - Article
C2 - 30472581
AN - SCOPUS:85056883953
SN - 0731-7085
VL - 164
SP - 475
EP - 480
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -