Enzyme-based amperometric galactose biosensors: a review

Prosper Kanyong, Francis D. Krampa, Yaw Aniweh, Gordon A. Awandare

Research output: Contribution to journalReview articlepeer-review

49 Citations (Scopus)

Abstract

This review (with 35 references) summarizes the various strategies used in biosensors for galactose, and their analytical performance. A brief comparison of the enzyme immobilization methods employed and the analytical performance characteristics of a range of galactose biosensors are first summarized in tabular form and then described in detail. Selected examples have been included to demonstrate the various applications of these biosensors to real samples. Following an introduction into the field that covers the significance of sensing galactose in various fields, the review covers biosensors based on the use of galactose oxidase, with a discussion of methods for their immobilization (via cross-linking, adsorption, covalent bonding and entrapment). This is followed by a short section on biosensors based on the use of galactose dehydrogenase. The conclusion section summarizes the state of the art and addresses current challenges.

Original languageEnglish
Pages (from-to)3663-3671
Number of pages9
JournalMicrochimica Acta
Volume184
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Keywords

  • Disposable sensors
  • Electrochemistry
  • Enzyme immobilization
  • Galactose dehydrogenase
  • Galactose oxidase
  • Glucose
  • Hydrogen peroxide
  • Modified electrodes
  • Redox mediators
  • Screen-printed electrodes

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