TY - JOUR
T1 - Elr-type proteins protect Xenopus Dead end mRNA from miR-18-mediated clearance in the soma
AU - Koebernick, Katja
AU - Loeber, Jana
AU - Arthur, Patrick Kobina
AU - Tarbashevich, Katsiaryna
AU - Pieler, Tomas
PY - 2010/9/14
Y1 - 2010/9/14
N2 - Segregation of the future germline defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.
AB - Segregation of the future germline defines a crucial cell fate decision during animal development. In Xenopus, germ cells are specified by inheritance of vegetally localized maternal determinants, including a group of specific mRNAs. Here, we show that the vegetal localization elements (LE) of Xenopus Dead end (XDE) and of several other germ-line-specific, vegetally localized transcripts mediate germ cell-specific stabilization and somatic clearance of microinjected reporter mRNA in Xenopus embryos. The part of XDE-LE critical for somatic RNA clearance exhibits homology to zebrafish nanos1 and appears to be targeted by Xenopus miR-18 for somatic mRNA clearance. Xenopus Elr-type proteins of the vegetal localization complex can alleviate somatic RNA clearance of microinjected XDE-LE and endogenous XDE mRNA. ElrB1 synergizes with Xenopus Dead end protein in the stabilization of XDE-LE mRNA. Taken together, our findings unveil a functional link of vegetal mRNA localization and the protection of germ-line mRNAs from somatic clearance.
KW - Development
KW - Germ cell
KW - MicroRNA
KW - RNA localization
KW - Vegetal
UR - http://www.scopus.com/inward/record.url?scp=77957990878&partnerID=8YFLogxK
U2 - 10.1073/pnas.1004401107
DO - 10.1073/pnas.1004401107
M3 - Article
C2 - 20805475
AN - SCOPUS:77957990878
SN - 0027-8424
VL - 107
SP - 16148
EP - 16153
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 37
ER -