TY - JOUR
T1 - Dual effect of taraxacum officinale leaves
T2 - Anticholinergic and inhibitory effect on inflammatory cells in ovalbumin-sensitized guinea-pigs
AU - Awortwe, C.
AU - Sackeyfio, A. C.
AU - Osei-Safo, Dorcas
AU - Bugyei, K. A.
AU - Asiedu-Gyekye, I. J.
PY - 2011/12/22
Y1 - 2011/12/22
N2 - Bronchospasm is an important characteristic of allergen-induced airway inflammation. Taraxacum officinale herb is believed to have several therapeutic effects including anti-inflammatory properties. The anticholinergic effect of the T. officinale leaves extract (TOLE) was determined in ovalbumin (OA)-sensitized guinea-pig trachea. The test drugs (TOLE or prednisolone) were administered orally 1 h prior to aerosolized 2% ovalbumin challenge. Isolated ovalbumin-sensitized guinea-pig tracheal was challenged with acetylcholine and OA in the absence and presence of TOLE. The blood samples were collected and neutrophils, lymphocytes and monocytes (eosinophils, basophils and macrophages) counts assayed. Prednisolone (2.5 mg/kg body weight (BW) orally) was used as reference standard. TOLE application showed significant antagonistic effect on contraction of trachea to both acetylcholine (35.10 ± 0.04) and OA (18.6 ± 1.5). Also, the extract reduced monocytes (0.62 ± 0.23), lymphocytes (3.4 ± 0.59) and neutrophils (3.65 ± 0.20) counts in OA-sensitized guinea-pigs. Thus, TOLE possesses anticholinergic and reduces neutrophil, eosinophil and basophil counts in ovalbumin-sensitized guinea-pigs.
AB - Bronchospasm is an important characteristic of allergen-induced airway inflammation. Taraxacum officinale herb is believed to have several therapeutic effects including anti-inflammatory properties. The anticholinergic effect of the T. officinale leaves extract (TOLE) was determined in ovalbumin (OA)-sensitized guinea-pig trachea. The test drugs (TOLE or prednisolone) were administered orally 1 h prior to aerosolized 2% ovalbumin challenge. Isolated ovalbumin-sensitized guinea-pig tracheal was challenged with acetylcholine and OA in the absence and presence of TOLE. The blood samples were collected and neutrophils, lymphocytes and monocytes (eosinophils, basophils and macrophages) counts assayed. Prednisolone (2.5 mg/kg body weight (BW) orally) was used as reference standard. TOLE application showed significant antagonistic effect on contraction of trachea to both acetylcholine (35.10 ± 0.04) and OA (18.6 ± 1.5). Also, the extract reduced monocytes (0.62 ± 0.23), lymphocytes (3.4 ± 0.59) and neutrophils (3.65 ± 0.20) counts in OA-sensitized guinea-pigs. Thus, TOLE possesses anticholinergic and reduces neutrophil, eosinophil and basophil counts in ovalbumin-sensitized guinea-pigs.
KW - Anticholinergic
KW - Blood cell counts
KW - Inflammation
KW - Taraxacum officinale
UR - http://www.scopus.com/inward/record.url?scp=84855376433&partnerID=8YFLogxK
U2 - 10.5897/AJPP11.616
DO - 10.5897/AJPP11.616
M3 - Article
AN - SCOPUS:84855376433
SN - 1996-0816
VL - 5
SP - 2613
EP - 2619
JO - African Journal of Pharmacy and Pharmacology
JF - African Journal of Pharmacy and Pharmacology
IS - 23
ER -