TY - JOUR
T1 - Diversity and evolution of the highly polymorphic tandem repeat LEI0258 in the chicken MHC-B region
AU - Chazara, Olympe
AU - Chang, Chi Sheng
AU - Bruneau, Nicolas
AU - Benabdeljelil, Khalid
AU - Fotsa, Jean Claude
AU - Kayang, Boniface B.
AU - Loukou, N'Goran E.
AU - Osei-Amponsah, Richard
AU - Yapi-Gnaore, Valentine
AU - Youssao, Issaka A.K.
AU - Chen, Chih Feng
AU - Pinard-Van Der Laan, Marie Hélène
AU - Tixier-Boichard, Michèle
AU - Bed'Hom, Bertrand
PY - 2013/6
Y1 - 2013/6
N2 - The chicken major histocompatibility complex (MHC) is located on the microchromosome 16 and is described as the most variable region in the genome. The genes of the MHC play a central role in the immune system. Particularly, genes encoding proteins involved in the antigen presentation to T cells. Therefore, describing the genetic polymorphism of this region is crucial in understanding host-pathogen interactions. The tandem repeat LEI0258 is located within the core area of the B region of the chicken MHC (MHC-B region) and its genotypes correlate with serology. This marker was used to provide a picture of the worldwide diversity of the chicken MHC-B region and to categorize chicken MHC haplotypes. More than 1,600 animals from 80 different populations or lines of chickens from Africa, Asia, and Europe, including wild fowl species, were genotyped at the LEI0258 locus. Fifty novel alleles were described after sequencing. The resulting 79 alleles were classified into 12 clusters, based on the SNPs and indels found within the sequences flanking the repeats. Furthermore, hypotheses were formulated on the evolutionary dynamics of the region. This study constitutes the largest variability report for the chicken MHC and establishes a framework for future diversity or association studies.
AB - The chicken major histocompatibility complex (MHC) is located on the microchromosome 16 and is described as the most variable region in the genome. The genes of the MHC play a central role in the immune system. Particularly, genes encoding proteins involved in the antigen presentation to T cells. Therefore, describing the genetic polymorphism of this region is crucial in understanding host-pathogen interactions. The tandem repeat LEI0258 is located within the core area of the B region of the chicken MHC (MHC-B region) and its genotypes correlate with serology. This marker was used to provide a picture of the worldwide diversity of the chicken MHC-B region and to categorize chicken MHC haplotypes. More than 1,600 animals from 80 different populations or lines of chickens from Africa, Asia, and Europe, including wild fowl species, were genotyped at the LEI0258 locus. Fifty novel alleles were described after sequencing. The resulting 79 alleles were classified into 12 clusters, based on the SNPs and indels found within the sequences flanking the repeats. Furthermore, hypotheses were formulated on the evolutionary dynamics of the region. This study constitutes the largest variability report for the chicken MHC and establishes a framework for future diversity or association studies.
KW - Chicken
KW - Evolution
KW - Gallus gallus
KW - LEI0258
KW - MHC
KW - Major histocompatibility complex
UR - http://www.scopus.com/inward/record.url?scp=84878013149&partnerID=8YFLogxK
U2 - 10.1007/s00251-013-0697-6
DO - 10.1007/s00251-013-0697-6
M3 - Article
C2 - 23529664
AN - SCOPUS:84878013149
SN - 0093-7711
VL - 65
SP - 447
EP - 459
JO - Immunogenetics
JF - Immunogenetics
IS - 6
ER -