TY - JOUR
T1 - Development of microsatellite markers for blue duiker (Cephalophus monticola) using next-generation sequencing and cross-amplification in other duikers
AU - Akomo-Okoue, Etienne François
AU - Inoue, Eiji
AU - Adenyo, Christopher
AU - Hayano, Azusa
AU - Inoue-Murayama, Miho
PY - 2013/9
Y1 - 2013/9
N2 - We developed nine microsatellite markers for duikers by next-generation sequencing using a muscle tissue sample of blue duiker (Cephalophus monticola). For blue duikers, the number of alleles ranged from 4 to 14 (mean 9.4), and the observed and expected heterozygosities were 0.333-0.833 (mean 0.716) and 0.625-0.878 (mean 0.817), respectively. Cumulative probability of identity was low (1.5 × 10-12), indicating that these markers are enough for individual discrimination. All nine markers were successfully amplified in three other duikers (C. dorsalis, C. ogilbyi and C. sylvicultor), and almost all loci were polymorphic. These markers will be useful for clarifying genetic structure of duikers.
AB - We developed nine microsatellite markers for duikers by next-generation sequencing using a muscle tissue sample of blue duiker (Cephalophus monticola). For blue duikers, the number of alleles ranged from 4 to 14 (mean 9.4), and the observed and expected heterozygosities were 0.333-0.833 (mean 0.716) and 0.625-0.878 (mean 0.817), respectively. Cumulative probability of identity was low (1.5 × 10-12), indicating that these markers are enough for individual discrimination. All nine markers were successfully amplified in three other duikers (C. dorsalis, C. ogilbyi and C. sylvicultor), and almost all loci were polymorphic. These markers will be useful for clarifying genetic structure of duikers.
KW - Duiker
KW - Genetic analysis
KW - Microsatellite markers
KW - Next-generation sequencing
UR - http://www.scopus.com/inward/record.url?scp=84881016533&partnerID=8YFLogxK
U2 - 10.1007/s12686-013-9906-8
DO - 10.1007/s12686-013-9906-8
M3 - Article
AN - SCOPUS:84881016533
SN - 1877-7252
VL - 5
SP - 779
EP - 781
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -