TY - JOUR
T1 - Development of 31 polymorphic microsatellite markers for guinea fowl (Numida meleagris) using next-generation sequencing technology
AU - Botchway, Princess K.
AU - Adenyo, Christopher
AU - Kayang, Boniface B.
AU - Hayano, Azusa
AU - Inoue-Murayama, Miho
PY - 2013/12
Y1 - 2013/12
N2 - Though efforts to domesticate guinea fowl (Numida meleagris) have been productive, both feral and wild populations continue to decline due to habitat fragmentation and heavy hunting, increasing the need to monitor and sustain this species. This study reports the first 31 original polymorphic microsatellite markers for guinea fowl by the next-generation sequencing technology. Observed (HO) and expected heterozygosities (HE) ranged from 0.033 to 1.000 (mean 0.396) and 0.033 to 0.799 (mean 0.419), respectively. Number of alleles ranged between 2 and 9 per locus (mean 3.387). Nine loci significantly deviated from Hardy-Weinberg Equilibrium (p < 0.05), after Bonferroni correction. Null allele frequency was less than 0.2 in all loci except locus Nmg22 (0.3). Results from this study can serve as baseline information for genetic diversity studies and also inform breeding strategies for the improvement and conservation in both domestic and wild populations of the species.
AB - Though efforts to domesticate guinea fowl (Numida meleagris) have been productive, both feral and wild populations continue to decline due to habitat fragmentation and heavy hunting, increasing the need to monitor and sustain this species. This study reports the first 31 original polymorphic microsatellite markers for guinea fowl by the next-generation sequencing technology. Observed (HO) and expected heterozygosities (HE) ranged from 0.033 to 1.000 (mean 0.396) and 0.033 to 0.799 (mean 0.419), respectively. Number of alleles ranged between 2 and 9 per locus (mean 3.387). Nine loci significantly deviated from Hardy-Weinberg Equilibrium (p < 0.05), after Bonferroni correction. Null allele frequency was less than 0.2 in all loci except locus Nmg22 (0.3). Results from this study can serve as baseline information for genetic diversity studies and also inform breeding strategies for the improvement and conservation in both domestic and wild populations of the species.
KW - Guinea fowl
KW - Microsatellites
KW - Next-generation sequencing
UR - http://www.scopus.com/inward/record.url?scp=84892869365&partnerID=8YFLogxK
U2 - 10.1007/s12686-013-9991-8
DO - 10.1007/s12686-013-9991-8
M3 - Letter
AN - SCOPUS:84892869365
SN - 1877-7252
VL - 5
SP - 1163
EP - 1165
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 4
ER -