Characteristics of hepatitis B virus in Ghana: Full length genome sequences indicate the endemicity of genotype E in West Africa

Tran Thien Tuen Huy, Koichi Ishikawa, William Ampofo, Taku Izumi, Akira Nakajima, Justina Ansah, John O. Tetteh, Nicholas Nii-Trebi, Simeon Aidoo, David Ofori-Adjei, Tetsutaro Sata, Hiroshi Ushijima, Kenji Abe

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) genotypes from A to H have distinct geographical distributions and have been shown to affect the clinical features as well as the course of the HBV infection. HBV genotype E has been found only in Africa. However, the complete genomes of this genotype, which were isolated mainly from West Africa, were available only for a few samples. In this study, five full-length genomes and seven other small S genes of HBV strains from Ghanaian blood donors were sequenced and investigated. Following phylogenetic analysis, all of the Ghanaian HBV strains were clustered closely in genotype E. All of the 12 small S genes showed the same characteristic of subtype ayw4. The complete genomes of the five Ghanaian strains showed marked similarity with each other and with the reported genotype E strains (96.7%-99.1%). Genotype E strains showed low intra-genotypic diversity (1.8%) and carried the conserved signature pattern in pre-S1 as well as in the full genome sequence. Of note, the finding of the G145R escape mutant in an unvaccinated Ghanaian blood donor might raise concern as to the ongoing nation-wide hepatitis B vaccination program in Ghana.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalJournal of Medical Virology
Volume78
Issue number2
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Keywords

  • Escape mutant
  • Full-length genome
  • Genotype E
  • Ghana
  • Hepatitis B virus

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