Bulk‐up synchronization of successive larval cohorts of Anopheles gambiae and Anopheles coluzzii through temperature reduction at early larval stages: effect on emergence rate, body size and mating success

Qaswa Zubair, Holly Matthews, Seynabou Sougoufara, Fatima Mujeeb, Simon Ashall, Fred Aboagye-Antwi, Frédéric Tripet

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: Malaria persists as a huge medical and economic burden. Although the number of cases and death rates have reduced in recent years, novel interventions are a necessity if such gains are to be maintained. Alternative methods to target mosquito vector populations that involve the release of large numbers genetically modified mosquitoes are in development. However, their successful introduction will require innovative strategies to bulk-up mosquito numbers and improve mass rearing protocols for Anopheles mosquitoes. Methods: The relationship between mosquito aquatic stage development and temperature was exploited so that multiple cohorts of mosquitoes, from separate egg batches, could be synchronized to ‘bulk-up’ the number of mosquitoes released. First instar larvae were separated into two cohorts: the first, maintained under standard insectary conditions at 27oC, the second subjected to an initial 5-day cooling period at 19oC. Results: Cooling of 1st instars slowed the mean emergence times of Anopheles coluzzii and Anopheles gambiae by 2.4 and 3.5 days, respectively, compared to their 27oC counterparts. Pupation and emergence rates were good (> 85 %) in all conditions. Temperature adjustment had no effect on mosquito sex ratio and adult fitness parameters such as body size and mating success. Conclusions: Bulk-up larval synchronization is a simple method allowing more operational flexibility in mosquito production towards mark-release-recapture studies and mass release interventions.

Original languageEnglish
Article number67
JournalMalaria Journal
Volume20
Issue number1
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Anopheles coluzzii
  • Anopheles gambiae
  • Larvae development
  • Mark release recapture studies
  • Mass rearing
  • Mosquito release programmes

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