TY - JOUR
T1 - Bacillus amyloliquefaciens D203 ameliorates rice growth and resistance to rice blast disease
AU - Mirara, Francis
AU - Dzidzienyo, Daniel Kwadjo
AU - Mwangi, Maina
N1 - Publisher Copyright:
© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2024
Y1 - 2024
N2 - This study evaluated the effects of Bacillus amyloliquefaciens D203 encapsulated in alginate-bentonite coating enriched with titanium nanoparticles on rice germination and Magnaporthe oryzae infection. Seed coating was achieved using the ionic gelation/extrusion method with modifications. The study investigated various concentrations of strain D203 on biomass and bacterial release from the beads, along with its influence on detached leaves. Results showed that treatment with a strain D203 cell suspension (2 × 107 CFU/ml) significantly enhanced root and shoot lengths, despite a slightly lower germination rate (90%) compared to the control. Treatment with a concentration of 1 × 107 proved to be optimum for growth because it maintained germination at 100% and boosted biomass growth of shoot and root by 49.57% and 16.44%, respectively. Colonization by strain D203, explored through seed coating and subsequent resuspension from roots and media, peaked in roots, indicating preferential root zone inhabitation. In vitro studies demonstrated strain D203’s efficacy against M. oryzae at 21 and 40 days, with the 1 × 107 CFU/ml spray significantly reducing disease symptoms. Strain D203 exhibited a preventive effect, especially in early growth stages. The encapsulated form of strain D203 had a control effect comparable to 40% Hexaconazole but less effective than 75% Tricyclazole. Uncoated and encapsulated B. amyloliquefaciens treatments significantly improved sterile rice plant growth over 21 days, with the encapsulated bead treatment surpassing both controls and free cell treatments by days 14 and 21, despite slower initial growth. Encapsulation presents a promising alternative, potentially matching the effectiveness of chemical fungicides, emphasizing the importance of environmentally friendly alternatives in sustainable disease management strategies.
AB - This study evaluated the effects of Bacillus amyloliquefaciens D203 encapsulated in alginate-bentonite coating enriched with titanium nanoparticles on rice germination and Magnaporthe oryzae infection. Seed coating was achieved using the ionic gelation/extrusion method with modifications. The study investigated various concentrations of strain D203 on biomass and bacterial release from the beads, along with its influence on detached leaves. Results showed that treatment with a strain D203 cell suspension (2 × 107 CFU/ml) significantly enhanced root and shoot lengths, despite a slightly lower germination rate (90%) compared to the control. Treatment with a concentration of 1 × 107 proved to be optimum for growth because it maintained germination at 100% and boosted biomass growth of shoot and root by 49.57% and 16.44%, respectively. Colonization by strain D203, explored through seed coating and subsequent resuspension from roots and media, peaked in roots, indicating preferential root zone inhabitation. In vitro studies demonstrated strain D203’s efficacy against M. oryzae at 21 and 40 days, with the 1 × 107 CFU/ml spray significantly reducing disease symptoms. Strain D203 exhibited a preventive effect, especially in early growth stages. The encapsulated form of strain D203 had a control effect comparable to 40% Hexaconazole but less effective than 75% Tricyclazole. Uncoated and encapsulated B. amyloliquefaciens treatments significantly improved sterile rice plant growth over 21 days, with the encapsulated bead treatment surpassing both controls and free cell treatments by days 14 and 21, despite slower initial growth. Encapsulation presents a promising alternative, potentially matching the effectiveness of chemical fungicides, emphasizing the importance of environmentally friendly alternatives in sustainable disease management strategies.
KW - Agriculture
KW - Agriculture & Environmental Sciences
KW - Agriculture and Food
KW - Bacillus amyloliquefaciens
KW - disease management
KW - Environmental Sciences
KW - germination
KW - magnaporthe oryzae
KW - Manuel Tejada, Universidad de Sevilla, Spain
KW - seed coating
UR - http://www.scopus.com/inward/record.url?scp=85197211891&partnerID=8YFLogxK
U2 - 10.1080/23311932.2024.2371943
DO - 10.1080/23311932.2024.2371943
M3 - Article
AN - SCOPUS:85197211891
SN - 2331-1932
VL - 10
JO - Cogent Food and Agriculture
JF - Cogent Food and Agriculture
IS - 1
M1 - 2371943
ER -