TY - JOUR
T1 - Association between Transcription Factor 7-like-2 Polymorphisms and Type 2 Diabetes Mellitus in a Ghanaian Population
AU - Obirikorang, Christian
AU - Adu, Evans Asamoah
AU - Anto, Enoch Odame
AU - Acheampong, Emmanuel
AU - Quaye, Lawrence
AU - Amoah, Brodrick Yeboah
AU - Annani-Akollor, Max Efui
AU - Kwakye, Aaron Siaw
AU - Fokuoh, Foster
AU - Appiah, Michael
AU - Nyarko, Eric Nana Yaw
AU - Aidoo, Freeman
AU - Adua, Eric
AU - Afrifa-Yamoah, Ebenezer
AU - Balmer, Lois
AU - Wang, Wei
N1 - Publisher Copyright:
© 2021 by the authors.
PY - 2021/12
Y1 - 2021/12
N2 - Type 2 diabetes mellitus (T2DM) has been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the association between rs12255372, rs7903146 in the TCF7L2 gene and T2DM in a Ghanaian population. A case-control study design was used for this study. A total of 106 T2DM patients and 110 control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and processed for: serum lipid analysis, plasma glucose estimation and plasma HbA1c estimation. Parts of the whole blood samples were used for DNA extraction using a modified salting-out method. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. The rs7903146 risk variant was significantly associated with 2.16 vs. 4.06 increased odds for T2DM in patients <60 years vs. ≥60 years. Both rs7903146 and rs12255372 were significantly associated with increased odds of T2DM in women, overweight/obese, T2DM negative family history (T2DM-NFH) and low-HDL-C. In a multivariate model, rs7903146 but not rs12255372 was significantly associated with 2.18, 5.01 and 2.25 increased odds of T2DM, under the codominant, recessive and additive model, respectively (p < 0.05). The association between rs7903146 and rs12255372 with T2DM is more highly associated in a subgroup—women and those with T2DM-NFH, yet who have cardiometabolic risk.
AB - Type 2 diabetes mellitus (T2DM) has been strongly associated with single nucleotide polymorphisms (SNPs) in the TCF7L2 gene. This study investigated the association between rs12255372, rs7903146 in the TCF7L2 gene and T2DM in a Ghanaian population. A case-control study design was used for this study. A total of 106 T2DM patients and 110 control participants were selected. Basic data collected included body mass index, blood pressure and socio-demographics. Fasting blood samples were collected and processed for: serum lipid analysis, plasma glucose estimation and plasma HbA1c estimation. Parts of the whole blood samples were used for DNA extraction using a modified salting-out method. Common and allele-specific primers were designed for genotyping using the Modified Tetra-Primer Amplification assay. Associations were evaluated using logistic regression models. The rs7903146 risk variant was significantly associated with 2.16 vs. 4.06 increased odds for T2DM in patients <60 years vs. ≥60 years. Both rs7903146 and rs12255372 were significantly associated with increased odds of T2DM in women, overweight/obese, T2DM negative family history (T2DM-NFH) and low-HDL-C. In a multivariate model, rs7903146 but not rs12255372 was significantly associated with 2.18, 5.01 and 2.25 increased odds of T2DM, under the codominant, recessive and additive model, respectively (p < 0.05). The association between rs7903146 and rs12255372 with T2DM is more highly associated in a subgroup—women and those with T2DM-NFH, yet who have cardiometabolic risk.
KW - TCF7L2
KW - cardiometabolic risk factors
KW - single nucleotide polymorphisms
KW - type 2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=85145216689&partnerID=8YFLogxK
U2 - 10.3390/sci3040040
DO - 10.3390/sci3040040
M3 - Article
AN - SCOPUS:85145216689
SN - 2413-4155
VL - 3
JO - Sci
JF - Sci
IS - 4
M1 - 40
ER -