TY - JOUR
T1 - Antierythropoietin Antibody Production Is Not Associated with Malaria and Malaria-Related Anaemia in Humans
AU - Addai-Mensah, Otchere
AU - Gyamfi, Daniel
AU - Amponsah, Francis Agyei
AU - Annani-Akollor, Max Efui
AU - Danquah, Kwabena Owusu
AU - Boateng, Lillian
AU - Owiredu, Eddie Williams
AU - Afriyie, Edward Y.
AU - Duneeh, Richard Vikpebah
AU - Asare, Renate
AU - Ofosu Ntiamoah, David
AU - Boateng, Richard
N1 - Publisher Copyright:
© 2019 Otchere Addai-Mensah et al.
PY - 2019
Y1 - 2019
N2 - Introduction. The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans. Methods. A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method. Results. Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032). Conclusion. Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia.
AB - Introduction. The pathophysiology of malaria-related anaemia is not fully understood although increased destruction of parasitized and nonparasitized erythrocytes, as well as inadequate erythropoiesis, has been proposed. Circulating antierythropoietin (anti-EPO) antibodies have also been implicated in malaria and malaria-related anaemia in mice. However, studies on this association have not been investigated in humans. This study therefore determined the prevalence of anti-EPO antibody production and assessed its association with malaria and malaria-related anaemia in humans. Methods. A total of 86 children aged 1-10 years (57 children with malaria serving as the case group and 29 healthy children serving as control), all residents of Duayaw Nkwanta, Ghana, were recruited for this case-control study. Venous blood was collected for thick and thin films for malaria microscopy, full blood count by automated haematology analyzer, and antierythropoietin antibody and erythropoietin estimation by sandwich ELISA method. Results. Out of the 86 participants recruited, only 3 (3.5%) were positive for anti-EPO antibody; 2.3% of the case group; and 1.2% of the control group. There was no association between the cases and the controls in the production of anti-EPO antibodies. Erythropoietin concentration was significantly higher in malaria-related anaemic subjects (p=0.032). Conclusion. Antierythropoietin antibodies are not associated with malaria infection and malaria-related anaemia in humans. Erythropoietin concentration is associated with malaria-related anaemia.
UR - http://www.scopus.com/inward/record.url?scp=85062336880&partnerID=8YFLogxK
U2 - 10.1155/2019/5398732
DO - 10.1155/2019/5398732
M3 - Article
C2 - 30894794
AN - SCOPUS:85062336880
SN - 2356-6140
VL - 2019
JO - Scientific World Journal
JF - Scientific World Journal
M1 - 5398732
ER -
