Analytical Methods in Pharmacoproteomics: A Systematic Review

Pharmacoproteomics is the proteomic analysis for discovering novel drug targets and studying drug metabolism, efficacy, and toxicity. This chapter aims to review analytical methods used in pharmacoproteomic studies systematically. A search was performed in Scopus, PubMed, and Web of Science online databases using appropriate search queries and reported as per the “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) guidelines. This review included sixty-seven peer-reviewed articles published between 2006 and 2023. About 72 drugs of diverse classes were studied with human or murine cell lines, parasitic strains, animal models, and human patient samples. Mass spectrometry was the primary analytical method used for pharmacoproteomic studies and sometimes in combination with immunoanalytical methods such as 1D SDS-PAGE and 2D SDS-PAGE. Most studies used label-free mass spectrometry (49/67), while others used labeling reagents like iTRAQ, SILAC, TMT, and ICAT. The pharmacoproteomic studies reported several differentially expressed proteins and unique outcomes about drug targets, mechanism of action, efficacy, and metabolism. This review identified mass spectrometry as the primary analytical method for pharmacoproteomic studies.