TY - JOUR
T1 - A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings
AU - Yalley, Akua K.
AU - Ahiatrogah, Selasie
AU - Kafintu-Kwashie, Anna A.
AU - Amegatcher, Gloria
AU - Prah, Diana
AU - Botwe, Akua K.
AU - Adusei-Poku, Mildred A.
AU - Obodai, Evangeline
AU - Nii-Trebi, Nicholas I.
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/10
Y1 - 2022/10
N2 - Infectious diseases significantly impact the health status of developing countries. Historically, infectious diseases of the tropics especially have received insufficient attention in worldwide public health initiatives, resulting in poor preventive and treatment options. Many molecular tests for human infections have been established since the 1980s, when polymerase chain reaction (PCR) testing was introduced. In spite of the substantial innovative advancements in PCR technology, which currently has found wide application in most viral pathogens of global concern, the development and application of molecular diagnostics, particularly in resource-limited settings, poses potential constraints. This review accessed data from sources including PubMed, Google Scholar, the Web of Knowledge, as well as reports from the World Health Organization’s Annual Meeting on infectious diseases and examined these for current molecular approaches used to identify, monitor, or investigate some neglected tropical infectious diseases. This review noted some growth efforts in the development of molecular techniques for diagnosis of pathogens that appear to be common in resource limited settings and identified gaps in the availability and applicability of most of these molecular diagnostics, which need to be addressed if the One Health goal is to be achieved.
AB - Infectious diseases significantly impact the health status of developing countries. Historically, infectious diseases of the tropics especially have received insufficient attention in worldwide public health initiatives, resulting in poor preventive and treatment options. Many molecular tests for human infections have been established since the 1980s, when polymerase chain reaction (PCR) testing was introduced. In spite of the substantial innovative advancements in PCR technology, which currently has found wide application in most viral pathogens of global concern, the development and application of molecular diagnostics, particularly in resource-limited settings, poses potential constraints. This review accessed data from sources including PubMed, Google Scholar, the Web of Knowledge, as well as reports from the World Health Organization’s Annual Meeting on infectious diseases and examined these for current molecular approaches used to identify, monitor, or investigate some neglected tropical infectious diseases. This review noted some growth efforts in the development of molecular techniques for diagnosis of pathogens that appear to be common in resource limited settings and identified gaps in the availability and applicability of most of these molecular diagnostics, which need to be addressed if the One Health goal is to be achieved.
KW - infectious diseases
KW - molecular diagnostics
KW - polymerase chain reaction
KW - tropical diseases
UR - http://www.scopus.com/inward/record.url?scp=85140631355&partnerID=8YFLogxK
U2 - 10.3390/cimb44100300
DO - 10.3390/cimb44100300
M3 - Review article
C2 - 36286015
AN - SCOPUS:85140631355
SN - 1467-3037
VL - 44
SP - 4367
EP - 4385
JO - Current Issues in Molecular Biology
JF - Current Issues in Molecular Biology
IS - 10
ER -